Environmental Presence and Genetic Characteristics of Carbapenemase-Producing Enterobacteriaceae from Hospital Sewage and River Water in the Philippines

Yuki Suzuki; Pearl Joy Nazareno; Ryuichi Nakano; Melisa Mondoy; Akito Nakano; Mark Philip Bugayong; Josie Bilar; Mauricio Perez; Emarld Julian Medina; Mariko Saito-Obata; Mayuko Saito; Kazutoshi Nakashima; Hitoshi Oshitani; Hisakazu Yano

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Environmental Presence and Genetic Characteristics of Carbapenemase-Producing Enterobacteriaceae from Hospital Sewage and River Water in the Philippines

Yuki Suzuki ^[1] ; Pearl Joy Nazareno ^[2] ; Ryuichi Nakano ^[1] ; Melisa Mondoy ^[2] ; Akiyo Nakano ^[1] ; Mark Philip Bugayong ^[2] ; Josie Bilar ^[2] ; Mauricio Perez ^[2] ; Emarld Julian Medina ^[2] ; Mariko Saito-Obata ^[3] ; Mayuko Saito ^[3] ; Kazutoshi Nakashima ^[4] ; Hitoshi Oshitani ^[3] ; Hisakazu Yano ^[1]
1. [1] Nara Medical University
  
  Nara Medical University
  
  Japón
2. [2] Research Institute for Tropical Medicine
  
  Research Institute for Tropical Medicine
  
  Santa Cruz, Filipinas
3. [3] Tohoku University
  
  Tohoku University
  
  Aoba-ku, Japón
4. [4] Daito Bunka University
  
  Daito Bunka University
  
  Japón
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Localización: Applied and Environmental Microbiology, ISSN 0099-2240, Vol. 86, Nº 2, 2020
Idioma: inglés
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Resumen
- Carbapenemase-producing Enterobacteriaceae (CPE) cause severe health care-associated infections, and their increasing prevalence is a serious concern. Recently, natural ecosystems have been recognized as important reservoirs of antibiotic resistance genes. We investigated the prevalence and genetic characteristics of CPE isolated from the environment (hospital sewage and river water) in the Philippines and found several CPE, including Escherichia coli and other species, with different carbapenemases. The most prevalent carbapenemase gene type was NDM, which is endemic in clinical settings. This study revealed that isolates belonging to carbapenemase-producing E. coli CC10 and K. pneumoniae sequence type 147 (ST147), which are often detected in clinical settings, were dominant in the natural environment. Our work here provides a report on the presence and characteristics of CPE in the environment in the Philippines and demonstrates that both hospital sewage and river water are contaminated by CPE strains belonging to clinically important clonal groups.