Staining of antinuclear antibodies and antibodies against removable nuclear antigens in connective tissue diseases

• José Enrique Oliva Menacho ^[2] ; Lester Domínguez-Huarcaya ^[3] ; Jorge Luis Arroyo-Acevedo ^[1] ; José Arturo Oliva-Candela ^[4] ; Marco Antonio García-Hjarles ^[2]
  1. [1] Universidad Nacional Mayor de San Marcos

     Universidad Nacional Mayor de San Marcos

     Perú

     
  2. [2] Cayetano Heredia Peruvian University, Lima, Peru
  3. [3] Faculty of Medicine of the University Norbert Wiener, Lima, Peru
  4. [4] Arzobispo Loayza National Hospital, Lima, Peru

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• Localización: Allergologia et immunopathologia: International journal for clinical and investigate allergology and clinical immunology, ISSN-e 1578-1267, ISSN 0301-0546, Vol. 48, Nº. 1, 2020, págs. 18-25
• Idioma: inglés
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• Resumen

  • Introduction and objectives Connective tissue diseases are inflammatory, autoimmune diseases and threaten quality of life. To determine the relationship between staining patterns of antinuclear antibodies and antibodies against extractable nuclear antigens in patients with connective tissue disease.

    Materials and methods Observational, basic, analytical and transversal study. Study conducted in the Immunology Service of the Arzobispo Loayza National Hospital between January 2017 and June 2017. We analyzed 291 samples of patients with CTD and for the detection of anti-nuclear antibody staining patterns, the immunological kit and observation with microscope of at 40X Immunofluorescence and for the detection of the antibodies against extractable nuclear antigens. The Immunoblot method was employed. Statistical analyses were carried out with the statistical package SPSS version 21 for Windows. We used the Pearson Chi-square test for the categorical variables, a value of p<0.05 was considered significant.

    Results There was a significant relationship p<0.05 of the homogeneous pattern, the mottled pattern with Anti-histones (p=0.000), Anti-nucleosomes (p=0.000), Anti-Ro 52 (p=0.000), Anti-SSA (p=0.001), Anti-SSB (p=0.003), Anti-dsDNA (p=0.000) with the Pearson Chi-square test. There was a significant relationship of p<0.05 of the centromeric pattern with Anti-Cenp B (p=0.000) with Fisher⿿s exact statistic.

    Conclusions There was a significant relationship between the anti-nuclear antibody staining patterns and the antibodies to the core extractable antigens in patients with systemic lupus erythematosus, Sjögren⿿s syndrome, Calcinosis, Raynaud⿿s phenomenon, esophageal Dysmotility, sclerodactyly and Telangiectasia (CREST), Scleroderma and Polymyositis.