Histochemical and morphometric evidences of the curative role of aqueous zest extract of Citrus sinensis on anti-neoplastic drug-induced testicular degeneration in animal models
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[1] University of Nigeria
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- Localización: European Journal of anatomy, ISSN-e 1136-4890, Vol. 22, Nº. 6, 2018, págs. 497-507
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
This study examined the curative effects of aqueous zest extract of Citrus sinensis and Cisplatin (CIS)-induced testicular degeneration. Sixteen male Wistar rats (10 to 12 weeks old) weighing 306-238 g were used in this study. The animals were divided as follows: Group A was treated orally with 2.5 ml/kg body weight/daily; Group B was treated with a single dose of 10 mg/kg body weight; Group C and D rats were given a single dose of 10 mg/kg body weight of cisplatin and then treated orally with 10 and 40 mg/kg body weight of aqueous zest extract of Citrus sinensis. The procedure lasted for 8 weeks.Results showed a significant (p< 0.05) decrease in final body weight, testis weight, testis weight/body weight ratio, normal sperm morphology (p<0.01) and a significant decrease in tubular diameter (p > 0.01), perimeter (p > 0.01 and) and length (p > 0.001), width (p >0.05) and increase (p > 0.05), germinal epithelia height, cross-sectional area, number of profiles per unit area and numerical density of seminiferous tubules. Rats that were treated with CIS alone without pre-treatment or post-treatment with extract showed marked degeneration and atrophied seminiferous tubules with absence of late stage germ cells. There was also a reduction in PAS-positive materials of the rats treated with Cisplatin. These parameters were however ameliorated in the groups that were post-treated with the aqueous zest extract of Citrus sinensis. This could have been as a result of its antioxidant and free radical scavenging potentials.
