The possible protective effect of Bougainvillea spectabilis leaves extract on estradiol valerate-induced polycystic ovary syndrome in rats (biochemical and histological study)

• Ayman M. Badawi ; Noha A. Ebrahim ^[1] ; Sahar B. Ahmed ; Aliaa A. Hassan ; Doaa M. Khaled ^[2]
  1. [1] Fayoum University

     Fayoum University

     Egipto

     
  2. [2] Helwan University

     Helwan University

     Egipto

     
• Localización: European Journal of anatomy, ISSN-e 1136-4890, Vol. 22, Nº. 6, 2018, págs. 461-469
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Polycystic ovary syndrome (PCOS) is a reproductive and metabolic disorder in which the level of oxidative elements in blood rises. Bougainvillea spectabilis is a plant with a potent antioxidant effect, since it contains flavonoids. The aim of this work is to explore the possible protective potency of Bougainvillea spectabilis leaves (BSL) extract on ovarian folliculogenesis using a rat model of estradiol valerate (EV) - induced PCOS. Thirty six mature female rats were divided into four groups: 1. Control group (six rats). 2. EV group (ten rats), singly injected with 2mg/kg EV subcutaneously. 3. BSL group (ten rats), given 100mg/kg BSL extract orally for 30 days. 4. EV + BSL group (ten rats), singly injected with EV subcutaneously and given 100mg/kg BSL extract orally for 30 days. Biochemical measurements of serum levels of estrogen, testosterone, LH, FSH, glucose, totals lipids, and total antioxidants were done using ELISA method. Histological (using hematoxylin & eosin and Masson's trichrome stains) and immunohistochemical (using anti cyclooxygenase 2 {COX 2}) studies were performed. This was followed by morphometric measurements and statistical analysis. PCOS caused massive primordial follicle loss and development of cystic follicles with increase in fibrosis, COX 2 immunoexpression. There was a significant reduction in LH, estrogen and glucose serum levels and increase in FSH and the antioxidant serum levels between the EV + BSL-extract-treated group compared to EV-treated group. Histomorphometric studies also showed the significant changes in the number of follicles and decreased cyst formation in the combined therapy group. There was a significant role of BSL extract in restoration of ovarian folliculogenesis and reduction of biochemical, histological and immunohistochemical changes in EV treated group.