Comparative analysis of the remineralization potential of CPP–ACP with Fluoride, Tri-Calcium Phosphate and Nano Hydroxyapatite using SEM/EDX – An in vitro study

Charisma Thimmaiah; Priya Shetty; Sowmya B. Shetty; Srikant Natarajan; Nithya-Annie Thomas

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Comparative analysis of the remineralization potential of CPP–ACP with Fluoride, Tri-Calcium Phosphate and Nano Hydroxyapatite using SEM/EDX – An in vitro study

Charisma Thimmaiah ^[2] ; Priya Shetty ^[1] ; Sowmya B. Shetty ^[1] ; Srikant Natarajan ^[3] ; Nithya-Annie Thomas ^[4]
1. [1] Rajiv Gandhi University of Health Sciences
  
  Rajiv Gandhi University of Health Sciences
  
  India
2. [2] MDS, Assistant Professor, Department of Pedodontics and Preventive Dentistry, Manipal College of Dental Sciences, Mangalore. Manipal Academy of Higher Education, Manipal, Karnataka India
3. [3] MDS, Professor and Head, Department of Oral Pathology and Microbiology. Manipal College of Dental Sciences, Mangalore. Manipal Academy of Higher Education, Manipal, Karnataka, India
4. [4] MDS, Senior Lecturer, Department of Pedodontics and Preventive Dentistry, Indira Gandhi institute of dental sciences, Nellikuzhi, Kothamanagalam, Kerala -India. Kerala University of health sciences
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Localización: Journal of Clinical and Experimental Dentistry, ISSN-e 1989-5488, Vol. 11, Nº. 12 (November), 2019, págs. 1120-1126
Idioma: inglés
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Resumen
- In recent years, the non-invasive management of non cavitated caries lesions using remineralization systems to repair the enamel have received more attention from the scientific community. Aim: To quantitatively evaluate the remineralization potential of Casein phosphopeptide-amorphous calcium phosphate-fluoride(CPP-ACPF), Tri-calcium phosphate(TCP) & Nano-hydroxyapatite(nHAP) using Scanning Electron Microscopy(SEM) and Energy dispersive X-ray Analysis(EDX).
  
  40 enamel specimens were prepared, and immersed in demineralising solution at a pH of 4.4 for 96 hours at 37°C, to induce artificial carious lesions. Remineralization was carried out for a period of 30 days using CPP-ACPF, TCP, nHAP. The specimens were evaluated for calcium and phosphorus content using SEM-EDX.
  
  The Ca/P mass % after remineralization was significantly higher with CPP-ACP-F and TCP-F followed by nHAP.
  
  CPP-ACP-F and TCP can promote significant remineralization of incipient carious lesions. These are excellent delivery vehicles available in a slow release amorphous form to localize calcium, phosphate and fluoride at the tooth surface.