A Novel Regulated Hybrid Promoter That Permits Autoinduction of Heterologous Protein Expression in Kluyveromyces lactis

• Hassan Sakhtah ^[2] ; Juliane Behler ^[1] ; Alana Ali-Reynolds ^[2] ; Thomas B. Causey ^[2] ; Saulius Vainauskas ^[2] ; Christopher H. Taron ^[2]
  1. [1] University of Freiburg

     University of Freiburg

     Stadtkreis Freiburg im Breisgau, Alemania

     
  2. [2] a New England Biolabs, Ipswich, Massachusetts, USA
• Localización: Applied and Environmental Microbiology, ISSN 0099-2240, Vol. 85, Nº 14, 2019
• Idioma: inglés
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• Resumen

  • The yeast Kluyveromyces lactis is an important host for the expression of recombinant proteins at both laboratory and industrial scales. However, the system lacks a tightly regulated promoter that permits controlled expression of heterologous proteins. In this study, we report the engineering of a highly regulated strong hybrid promoter (termed P350) for use in K. lactis. P350 is tightly repressed by glucose or glycerol in the medium but strongly promotes gene expression once the carbon source has been consumed by the cells. This feature permits heterologous protein expression to be “autoinduced” at any scale without the addition of a gratuitous inducer molecule or changing feed solutions.