micro RNA Detection in Blood, Urine, Semen, and Saliva Stains After Compromising Treatments
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Layne, Tiffany R. [1] ; Green, Raquel A. [1] ; Lewis, Carolyn A. [1] ; Nogales, Francy [1] ; Dawson Cruz, Tracey C. [1] ; Zehner, Zendra E. [1] ; Seashols‐Williams, Sarah J.
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[1] Virginia Commonwealth University
Virginia Commonwealth University
Estados Unidos
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- Localización: Journal of forensic sciences, ISSN-e 1556-4029, ISSN 0022-1198, Vol. 64, Nº. 6, 2019, págs. 1831-1837
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
Evaluation of microRNA (miRNA) expression as a potential method for forensic body fluid identification has been the subjectof investigation over the past several years. Because of their size and encapsulation within proteins and lipids, miRNAs are inherently less sus-ceptible to degradation than other RNAs. In this work, blood, urine, semen, and saliva were exposed to environmental and chemical conditionsmimicking sample compromise at the crime scene. For many treated samples, including 100% of blood samples, miRNAs remained detectable,comparable to the untreated control. Sample degradation varied by body fluid and treatment, with blood remarkably resistant, while semen andsaliva are more susceptible to environmental insult. Body fluid identification using relative miRNA expression of blood and semen of theexposed samples was 100% and 94%, respectively. Given the overall robust results herein, the case is strengthened for the use of miRNAs as amolecular method for body fluid identification.
