Resumen de Potential Forensic Application of Receptor for Advanced Glycation End Products (RAGE) as a Novel Biomarker for Estimating Postmortem Interval
Studies which estimate the postmortem interval (PMI) based on diatoms or bacterial communities have been well-conducted.However, predicting the PMI based on microorganisms has limited accuracy. The objective of this study was to discover specific biomarkersfor estimating the PMI for drowning. Thein vivoexperimental model consisted of rats classified into nine groups (seven drowned groups fromDay 1 to Day 7 and two control groups). Their lung tissues were analyzed for the receptor for advanced glycation end products (RAGE)mRNA expression determined by qRT-PCR. RAGE protein levels were determined by Western blotting and immunohistochemical (IHC) stain-ing. RAGE mRNA and protein levels gradually degraded over time. Immunostaining analysis revealed that RAGE in the Day 1 sample wassignificantly higher than that in the Day 6 or Day 7 samples. These results indicate that RAGE in drowned lungs could be a potential biomar-ker for the determination of the PMI after drowning
