Development and Validation of a Method Using Dried Oral Fluid Spot to Determine Drugs of Abuse

• Jacques, Ana Laura Bemvenuti ^[1] ; Santos, Maíra Kerpel dos ^[1] ; Limberger, Renata Pereira ^[1]
  1. [1] Universidade Federal do Rio Grande do Sul

     Universidade Federal do Rio Grande do Sul

     Brasil

     
• Localización: Journal of forensic sciences, ISSN-e 1556-4029, ISSN 0022-1198, Vol. 64, Nº. 6, 2019, págs. 1906-1912
• Idioma: inglés
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• Resumen

  • A liquid chromatography–mass spectrometry method using dried oral fluid spots was developed and validated for the simulta- neous quantification of cocaine, benzoylecgonine, cocaethylene, amphetamine, and 3,4-methylenedioxymethamphetamine. The oral fluid was applied to a Whatman 903 grade paper and submitted to a drying time of 2.5 h. The extraction procedure was optimized by chemometric approach using simplex centroid design. Spots were extracted with a mixture of acetonitrile, buffer, and methanol. Calibration curves covered a linear concentration range of 40–500 ng/mL. Validation parameters of linearity, precision, accuracy, selectivity, carryover, matrix effects, and stability were evaluated and showed satisfactory results. Spot homogeneity was also satisfactory, with less than 15% of deviation from nominal concentration. Spot volume did not influence accuracy when less than 100 lL of the sample was applied to the spot. The validation of the proposed method suggests a potential application in different scenarios in toxicology.