Validation of microRNA expression profile in Oral Lichenoid Disease through cytological samples

• Amaia Setién-Olarra ^[1] ; Xabier Marichalar-Mendia ^[1] ; Juan-Alonso Fernández-Pacheco ^[3] ; Marcos Fernández-Barriales-López ^[3] ; María-Luisa Gainza-Cirauqui ^[2] ; José-Manuel Aguirre-Urizar ^[1]
  1. [1] Universidad del País Vasco/Euskal Herriko Unibertsitatea

     Universidad del País Vasco/Euskal Herriko Unibertsitatea

     Leioa, España

     
  2. [2] University of Malta

     University of Malta

     Malta

     
  3. [3] Servicio de Cirugía Maxilofacial. Hospital Universitario Araba (Osakidetza. OSI Araba). Vitoria-Gazteiz, Spain

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• Localización: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, ISSN-e 1698-6946, Vol. 24, Nº. 5 (September), 2019
• Idioma: inglés
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• Resumen

  • To validate oral exfoliative cytology in the analysis of the microRNA expression profile in Oral Lichenoid Disease (OLD).

    The expression of 13 microRNAs identified and presented by our group in a previous study was analyzed in 26 cases, 16 diagnosed as OLD and 10 controls with no oral mucosal pathology. Cytological samples from the oral mucosa obtained using an Orcellex toothbrush were analyzed using RT-qPCR and TaqMan microRNA assays.

    The aberrant expression was validated for 2 microRNAs (miR-146a-5p and miR-7-1-3p) of those previously recognized in the biopsy study.

    This is the first time that oral exfoliative cytology is validated in a study of the alterations of the expression of microRNAs in OLD. The alteration of miR-146a and miR-7 compared to controls was validated. These microRNAs are associated with both inflammatory and carcinogenic phenomena that are involved in the etiopathogenesis of this potentially malignant oral disorder.