Nanosecond pulsed electric field inhibits malignant melanoma growth by inducing the change of systemic immunity

• Xingxing Zhang ^[1] ; Yunxia Zhang ^[1] ; Jian Chen ^[3] ; Yuyan Wu ^[1] ; Jue Zhang ^[2] ; Jing Wang ^[1]
  1. [1] Lanzhou University

     Lanzhou University

     China

     
  2. [2] Peking University

     Peking University

     China

     
  3. [3] Prof. The First Hospital of Lanzhou University, 1 Donggang Western Road, Lanzhou Gansu 730000, China

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• Localización: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, ISSN-e 1698-6946, Vol. 24, Nº. 4 (July), 2019
• Idioma: inglés
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• Resumen

  • Nanosecond pulsed electric fields (nsPEFs) showed an inhibitory effect on proliferation of malignant melanoma. In this study, the growth of melanoma were inhibited by changing the systemic immunity.

    C57BL/6 mice with B16 malignant were exposed to 200 pulses of 100 ns duration, 30kV/cm. The mice were executed four days later. T lymphocyte has been extracted from spleen. Cell viability was evaluated by CCK-8 assay. CD3+CD4+ T cells, CD3+CD8+ T cells, regulatory T cells (Treg) and myeloid-derived suppressor cells (MDSC) were analyzed by flow cytometry. TNF-α, IL-2, IL-10, TGF-β, IFN– γ levels in supernatants were assessed by ELISA.

    C57 malignant melanoma model were established successfully. After the treatment of nsPEFs(30 kV/cm 100 ns 200p), the numbers of T lymphocytes were increased.CD3+ CD4+ T cells changed from 48% to 51.2%;CD3+CD8+T lymphocytes increased from 39.6% to 40.4%.Treg cells reduced from 4.3% to 2.4%,MDSC decreased by 39.0% to 19.7% . In addition, the level of TNF-α, IL-2 were increased (P< 0.05) and the level of IL-10 were decreased (P<0.05) and the level of TGF-β and IFN-γ remained stable (P>0.05).

    Tumor growth can be effectively inhibited by nsPEFs in vivo, which activate targets of immune respones, accumulation of inflammatory cells and immune cytokines.