Techniques for the study of the mineralization of -P-, contained in the -DNA-, by soil bacteria (1)

• Autores: J.M. Barea, R. Azcon, V. Callao
• Localización: Ars pharmaceutica, ISSN 0004-2927, ISSN-e 2340-9894, Vol. 11, Nº. 5-6, 1970, págs. 303-310
• Idioma: español
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• Resumen

  • A plate technique to investigate the ability for P liberation from DNA, by soil bacteria, has been studied.DNA, in colloidal suspension, (pH= 7-7,1), is added to a basal medium making it opaque.Detection of desoxirribonuclease producing organisms was achieved by halos obtained (photography).Halo formation must be estimated as a proof of P liberation from DNA, since it was confirmed, by checking of the same bacteria in another technique, (also described in this paper), using a basal liquid medium with DNA as sole phosphorus source.