Extraction of DNA from Skeletonized Postcranial Remains: A Discussion of Protocols and Testing Modalities

• Edson, Suni M. ^[1]
  1. [1] Flinders University

     Flinders University

     Australia

     
• Localización: Journal of forensic sciences, ISSN-e 1556-4029, ISSN 0022-1198, Vol. 64, Nº. 5, 2019, págs. 1312-1323
• Idioma: inglés
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• Resumen

  • This paper provides a retrospective of the DNA analysis performed by the Armed Forces Medical Examiner–Armed ForcesDNA Identification Laboratory between 1990 and 2018. Over 13,000 postcranial osseous materials, comprised of wartime losses from WorldWar II, the Korean War, and South-East Asia, were examined by the following: mitochondrial DNA sequencing, a modified AmpFlSTR Yfi-lerTM, AmpFlSTR MiniFilerTM, PowerPlex Fusion, or NGS. Four different DNA extraction protocols were used: incomplete demineralizationcoupled with an organic purification; complete demineralization with an organic purification; complete demineralization with an inorganicpurification using QIAquick PCR Purification Kit; and a protocol designed specifically for use with next-generation sequencing. In general,complete demineralization coupled with an organic purification was the optimal extraction protocol for sequencing of mitochondrial DNA,regardless of the osseous element tested. For STR testing, demineralization paired with an inorganic purification provided optimum results,regardless of kit used or osseous element tested.