Estimating Individual Contributions to Complex DNA SNP Mixtures.,

• Ricke, Darrell O. ; Fremont‐Smith, Philip ; Watkins, James ^[1] ; Stankiewicz, Sara ^[1] ; Boettcher, Tara ^[1] ; Schwoebel, Eric ^[1]
  1. [1] Massachusetts Institute of Technology

     Massachusetts Institute of Technology

     City of Cambridge, Estados Unidos

     
• Localización: Journal of forensic sciences, ISSN-e 1556-4029, ISSN 0022-1198, Vol. 64, Nº. 5, 2019, págs. 1468-1474
• Idioma: inglés
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• Resumen

  • High-throughput sequencing (HTS) of large panels of single nucleotide polymorphisms (SNPs) provides an alternative or com-plimentary approach to short tandem repeats (STRs) panels for the analysis of complex DNA mixture forensic samples. For STRs, methods toestimate individual contribution concentrations compare capillary electrophoresis peak heights, peak areas, or HTS allele read counts within amixture. This article introduces three approaches (mean, median, and slope methods) for estimating individual DNA contributions to forensicmixtures for HTS/massively parallel sequencing (MPS) SNP panels. For SNPs, the major:minor allele ratios or counts, unique to each contribu-tor, were compared to estimate contributor proportion within the mixture using the mean, median, and slope intercept for these alleles. The esti-mates for these three methods were typically within 5% of planned experimental contributions for defined mixtures.