Versatile Oxidase and Dehydrogenase Activities of Bacterial Pyranose 2-Oxidase Facilitate Redox Cycling with Manganese Peroxidase In Vitro

• Peter L. Herzog ^[1] ; Leander Sützl ^[1] ; Beate Eisenhut ^[1] ; Daniel Maresch ^[2] ; Dietmar Haltrich ^[1] ; Christian Obinger ^[2] ; Clemens K. Peterbauer ^[1]
  1. [1] a Department of Food Science and Technology, BOKU—University of Natural Resources and Life Sciences, Vienna, Austria
  2. [2] b Department of Chemistry, BOKU—University of Natural Resources and Life Sciences, Vienna, Austria
• Localización: Applied and Environmental Microbiology, ISSN 0099-2240, Vol. 85, Nº 13, 2019
• Idioma: inglés
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• Resumen

  • Establishment of a mechanistic synergism between pyranose oxidase and (manganese) peroxidases represents a vital step in the course of elucidating microbial lignin degradation. Here, the comprehensive characterization of a bacterial pyranose 2-oxidase from Kitasatospora aureofaciens is of particular interest for several reasons. First, the phylogenetic analysis of putative pyranose oxidase genes reveals a widespread occurrence of highly similar enzymes in bacteria. Still, there is only a single report on a bacterial pyranose oxidase, stressing the need of closing this gap in the scientific literature. In addition, the relatively small K. aureofaciens proteome supposedly supplies a limited set of enzymatic functions to realize lignocellulosic biomass degradation. Both enzyme and organism therefore present a viable model to study the mechanisms of bacterial lignin decomposition, elucidate physiologically relevant interactions with specialized peroxidases, and potentially realize biotechnological applications.