Resumen de B subset cells in patients with chronic granulomatous disease in a Mexican population
C.F. Pozo Beltrán, M.A. Suárez Gutiérrez, Marco Antonio Yamazaki-Nakashimada, Isabel Medina Vera, F. Saracho Weber, A.P. Macías Robles, M.N. Guzmán Martínez, Elsy Maureen Navarrete Rodríguez, Blanca Estela del Río Navarro, Sara Elva Espinosa Padilla, L. Blancas Galicia
Introduction Chronic granulomatous disease (CGD) is a disorder of phagocyte function, characterized by pyogenic infections and granuloma formation caused by defects in NADPH oxidase complex activity. Although the effect of CGD mainly reflects the phagocytic compartment, B cell responses are also impaired in patients with CGD.
Materials and methods Flow cytometric analysis was performed on peripheral blood samples from 35 CGD patients age-matched with healthy controls (HC). The target cells of our study were the naive (IgD+/CD27−), memory (IgD−/CD27+), and B1a (CD5+) cells. Immunoglobulins (Igs) were also measured. This study was performed in a Latin American cohort.
Results We found significantly higher levels of naive B cells and B1a cells, but lower levels of memory B cells were found in CGD patients compared to HC. There was no significant difference of cell percentages per inheritance type.
Discussion Our findings suggest that the deficiency of NADPH oxidase components can affect the differentiation of naive B cells to memory B cells. Consequently, memory cells will be low, which also influenced the expression of CD27 in memory B cells and as a result, the percentage of naive cells increases. An altered phenotype of B lymphocytes in CGD patients may contribute to the opportunistic infections and autoimmune disorders that are seen in this disease.
