Importance of Poly-3-Hydroxybutyrate Metabolism to the Ability of Herbaspirillum seropedicae To Promote Plant Growth

• Luis Paulo Silveira Alves ^[1] ; Fernanda Plucani do Amaral ^[2] ; Daewon Kim ^[2] ; Maritza Todo Bom ^[1] ; Manuel Piñero Gavídia ^[1] ; Cícero Silvano Teixeira ^[1] ; Fernanda Holthman ^[1] ; Fabio de Oliveira Pedrosa ^[1] ; Emanuel Maltempi de Souza ^[1] ; Leda Satie Chubatsu ^[1] ; Marcelo Müller-Santos ^[1] ; Gary Stacey ^[2]
  1. [1] Universidade Federal do Paraná

     Universidade Federal do Paraná

     Brasil

     
  2. [2] University of Missouri

     University of Missouri

     Township of Columbia, Estados Unidos

     
• Localización: Applied and Environmental Microbiology, ISSN 0099-2240, Vol. 85, Nº 6, 2019
• Idioma: inglés
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• Resumen

  • The application of bacteria as plant growth promoters is a sustainable alternative to mitigate the use of chemical fertilization in agriculture, reducing negative economic and environmental impacts. Several plant growth-promoting bacteria synthesize and accumulate the intracellular polymer polyhydroxybutyrate (PHB). However, the role of PHB in plant-bacterium interactions is poorly understood. In this study, applying the C4 model grass Setaria viridis and several mutants in the PHB metabolism of the endophyte Herbaspirillum seropedicae yielded new findings on the importance of PHB for bacterial colonization of S. viridis roots. Taken together, the results show that deletion of genes involved in the synthesis and degradation of PHB reduced the ability of the bacteria to enhance plant growth but with little effect on overall root colonization. The data suggest that PHB metabolism likely plays an important role in supporting specific metabolic routes utilized by the bacteria to stimulate plant growth.