Resumen de Spiked Virus Level Needed To Correctly Assess Enteric Virus Recovery in Water Matrices

Qiaozhi Li, Yuanyuan Qiu, Xiao L. Pang, Nicholas J. Ashbolt

• The performance of procedures for pathogen log reduction is at the heart of new risk-based guidance/regulation globally, yet the methods for undertaking assessments of pathogen recovery are not standardized despite their fundamental impacts on assessing log reductions. Here we describe the level of spiking agent(s) that is necessary to correctly assess spiked pathogen/surrogate recovery with whatever method is deployed. The significance of our research lies in identifying the importance of the amount of spiking agents for reducing uncertainty in recovery estimates, which will allow the development of a recommendation for spiking experiments, proactively applying this understanding.