Resumen de Purificación de mastocitos de fluido peritoneal de rata utilizando gradientes continuos de Ficoll
Luis Miguel Botana López, Joaquín Espinosa Gallego, María Pilar Fernández Otero
A method for mast cells purification from rat peritoneal fluid is described. The method consists of a continuous Ficoll gradient between 20 and 22,5% (w/v) Ficoll and the cells are obtained with an 88% retrieval. Purity and viability were of 95% and 97% respectively. The cells so purified were functional against the compound 48/80 and the spontaneous secretion value was less than 8%.
