Resumen de Pesquisa de anticuerpos seroneutralizantes para pestivirus y herpesvirus en ovinos, caprinos y camélidos sudamericanos de Chile

M.O. Celedón, A Sandoval, J. Droguett, R. Calfio, L. Ascencio, J. Pizarro, C. Navarro

• español

  Se investigó la presencia de anticuerpos por dilución punto final seroneutralizante (DPFSN) para pestivirus (cepa NADL del virus de la diarrea viral bovina) y para herpesvirus (cepa Los Angeles del virus herpes bovino 1), en muestras de sueros de 321 ovinos, 322 caprinos, 74 alpacas (Lama pacos ), 43 llamas (Lama glama ), 48 guanacos (Lama guanicoe ) y 34 vicuñas (Vicugna vicugna ), procedentes de diferentes regiones del país. Para pestivirus se detectó reacción serológica positiva en 60 (18,7%) ovinos, en 21 (6,5%) caprinos, en 8 (10,8%) alpacas y en 6 (14%) llamas. Los guanacos y vicuñas fueron seronegativos para pestivirus. Para herpesvirus, la seropositividad se obtuvo en 8 (2,5%) ovinos y 62 (19,3%) caprinos. No se detectaron anticuerpos para herpesvirus en las muestras de camélidos. Según la distribución geográfica los mayores porcentajes de positividad resultaron ser: para pestivirus en ovinos de 2/3 predios de la XII Región (con positividad de 66,7% y 82,1%), con rangos de títulos de 16 a 710 y, para herpesvirus en caprinos de 5/6 predios de la IV Región (con positividad de 4,2%, 13,3%, 28,6%, 61,5% y 66,7%), con rango de títulos de 2 a 45. Las alpacas y llamas serorreaccionantes a pestivirus se encontraban ubicadas en la Región Metropolitana, en confinamiento en conjunto con otras especies de rumiantes, en cambio que las especies silvestres, guanacos y vicuñas fueron muestreadas en sus lugares de origen. Se confirma que en Chile existe infección por pestivirus en ovinos, caprinos, llamas y alpacas y por herpesvirus en ovinos y caprinos

• English

  Microtitration serum virus-neutralization tests were used to determine antibody titres for pestivirus: bovine viral diarrhea virus (NADL strain) and herpesvirus: bovine herpes virus 1 (Los Angeles strain) in 321 sheep, 322 goats, 74 alpacas (Lama pacos ), 43 llamas (Lama glama ), 48 guanacos (Lama guanicoe ) and 34 vicuñas (Vicugna vicugna ), from several Regions of Chile. Antibodies to pestivirus were found in 60 (18,7%) sheep, in 21 (6,5%) goats, in 8 (10,8%) alpacas and in 6 (14%) llamas. The guanacos and vicuñas did not have antibodies to pestivirus. Antibodies to herpesvirus were found in 8 (2,5%) sheep and in 62 (19,3%) goats. The alpacas, llamas, guanacos and vicuñas did not antibodies to herpesvirus. From the seropositive animals to pestivirus, the sheep flocks were located in the Metropolitan Region and XII Region of Chile with 7,1% to 82,1% positive serum samples, with titres between 11 to 1024, and 5 (22,7%) positive flocks; The goat flocks were located in the IV Region and the Metropolitan Region with 6,7% to 100% positive serum samples, with titers between 4 to 512, and 3 (13,6%) positive flocks. The alpacas and llamas flocks were located in the Metropolitan Region (where they live with other ruminants). Alpacas 10,8% serum samples from 2 flocks and 14% serum sample of llamas from 1 flock, were positive, with titers between 32 to 2048. From the seropositive animals to herpesvirus, only one sheep flock was positive and it was located in VI Region of Chile with 28,6% positive serum samples, with titers between 2 to 5,6; The goat flocks positive to herpesvirus were located in the IV Region and the Metropolitan Region with 4,2% to 66,7% positive serum samples, with titers between 2 to 45, and 14 (63,6%) positive flocks. The serum samples from guanacos and vicunas were obtained from their natural Regions, guanacos from the IV and XII Regions and vicuñas from the I Region. The findings of our study confirm that pestivirus infections of sheep, goats, alpacas and llamas and herpesvirus infections of sheep and goats occur in Chile