Internal Validation of Rapid HIT ID ACE Sample Cartridge and Assessment of the EXT Sample Cartridge*

• Amick, Gray D. ^[1] ; Swiger, Roy R. ^[2]
  1. [1] Richland County Sheriff's Department (RCSD) Forensics Laboratory.Estados Unidos
  2. [2] Scientific Management Group Inc..Estados Unidos
• Localización: Journal of forensic sciences, ISSN-e 1556-4029, ISSN 0022-1198, Vol. 64, Nº. 3, 2019, págs. 857-868
• Idioma: inglés
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• Resumen

  • A new rapid DNA solution, the RapidHIT ID, can accommodate two different sample cartridges, ACE, for the analysis of a single swab and EXT, for the analysis of DNA extracts. An efficient internal validation designed for low-throughput rapid DNA is described. An evaluation of the EXT sample cartridge is also described. Each cartridge generated profiles with sufficient data quality to meet CODIS eligibility in fewer than 120 min. The results exhibited 100% correlation when compared to conventional DNA typing methods. Precision, reproducibility, stochastic, mixture, and contamination experiments produced expected results. Sensitivity of the ACE sample cartridge was acceptable for buccal swab analysis. The sensitivity of the EXT sample cartridge is discussed. The ACE validation and the EXT evaluation utilized a minimalist, cost-saving, efficient design to generate a validated RapidHIT ID instrument capable of producing genetic profiles from both extracted forensic DNA samples and buccal swab samples within 120 min.