Radioimmunoassay for melatonin and its application to fowl pineal research

• Autores: Agustín Francisco Sigüenza Molina, J. M. Recio, Maria Teresa Agapito Serrano
• Localización: Revista española de Fisiología, ISSN 0034-9402, Vol. 44, Nº. 2, 1988, págs. 221-226
• Idioma: inglés
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• Resumen

  • A radioimmunoassay for melatonin has been developed after raising anti-melatonin antibodies in rabbit. Melatonin was extracted from serum or pineal gland of chickens (Gallus domesticus). The radioimmunoassay was performed by using 3H-melatonin as tracer. The standard curve covered the range 0.022-0.345 pmol/vial and the KD value for melatonin was estimated at 1.37 x 10(10) l/mol. The antiserum specificity has been analysed, none of the common melatonin analogues influencing this method of melatonin measurement. The intra-assay variability was 7.2% for serum samples and 8.6% for pineal extract. The inter-assay variability for this biological sample was 15.3% and 6.4% respectively.