Resumen de In silico analysis of transferable QepA variants and related chromosomal efflux pump
Marina Fernández Torres, Itziar Angulo López, Patricia Ruiz Bueno, Marta González Martinez, Carlos Ruiz de Alegría Puig, Jesús Rodríguez Lozano, Jesús Agüero Balbín, Javier Crespo García, Jorge Calvo Montes
- español
Objetivos. El objetivo del presente estudio fue detectar la presencia en GenBank de variantes de QepA no descritas.
Materiales y métodos. Las secuencias de ADN y aminoácidos de QepA1 se compararon en GenBank. Sólo se consideraron las identidades >80%. Las secuencias sintéticas o parciales no se incluyeron en los análisis.
Resultados. Los resultados mostraron la presencia de 10 variantes de QepA, 6 de ellas sin designación alélica.
Adicionalmente se observaron elevados niveles de identidad con bombas MSF cromosomales del genero Pseudorhodoferax y de otras Comamonadaceae.
Conclusiones. Se reporta la presencia en GenBank de variantes no descritas de QepA, así como la presencia en miembros del orden Burkholderiales de secuencias relacionadas.
- English
Objectives. The present study aimed to detect the presence of undescribed QepA variants in GenBank records.
Material and methods. The DNA and amino acid sequences of QepA1 were compared with what is present in GenBank. Only annealings with a >80% identity were considered. No synthetic or partial sequences were included in the analyses.
Results. The results showed the presence of 10 different QepA variants, 6 of them which were previously non-designated as specific allelic variants. In addition, high identity levels with chromosomal MSF efflux pumps belonging to microorganisms of the Pseudorhodoferax genus and other Comamonadaceae were detected.
Conclusions. The presence of undescribed QepA variants in GenBank is reported and the presence of related sequences among members of Burkholderiales order is described.
