Centromeres license the mitotic condensation of yeast chromosome arms

• Tom Kruitwagen ^[1] ; Pierre Chymkowitch ^[2] ; Annina Denoth Lippuner ^[3]
  1. [1] Swiss Federal Institute of Technology in Zurich

     Swiss Federal Institute of Technology in Zurich

     Zürich, Suiza

     
  2. [2] Oslo University Hospital

     Oslo University Hospital

     Noruega

     
  3. [3] University of Zurich

     University of Zurich

     Zürich, Suiza

     

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• Localización: Cell, ISSN 0092-8674, Vol. 175, Nº. 3, 2018, págs. 780-795
• Idioma: inglés
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  • During mitosis, chromatin condensation shapes chromosomes as separate, rigid, and compact sister chromatids to facilitate their segregation. Here, we show that, unlike wild-type yeast chromosomes, non-chromosomal DNA circles and chromosomes lacking a centromere fail to condense during mitosis. The centromere promotes chromosome condensation strictly in cis through recruiting the kinases Aurora B and Bub1, which trigger the autonomous condensation of the entire chromosome. Shugoshin and the deacetylase Hst2 facilitated spreading the condensation signal to the chromosome arms. Targeting Aurora B to DNA circles or centromere-ablated chromosomes or releasing Shugoshin from PP2A-dependent inhibition bypassed the centromere requirement for condensation and enhanced the mitotic stability of DNA circles. Our data indicate that yeast cells license the chromosome-autonomous condensation of their chromatin in a centromere-dependent manner, excluding from this process non-centromeric DNA and thereby inhibiting their propagation.