Liquid Chromatography Mass Spectrometry (LC-MS) analysis in determining the saliva protein of orthodontic patients during retention phase
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Noor-Hidayah Awang-Kechik [1] ; Rohana Ahmad [3] ; Saeid-Reza Doustjalali [2] ; Negar-Shafiei Sabet [2] ; Aida-Nur-Ashikin Abd-Rahman [1]
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[1] Universiti Teknologi MARA
Universiti Teknologi MARA
Malasia
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[2] SEGi University
SEGi University
Malasia
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[3] Associate Professor, Faculty of Dentistry, Universiti Teknologi MARA, and Integrative Pharmacogenomics Institute, Puncak Alam Campus, Universiti Teknologi MARA, Malaysia
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- Localización: Journal of Clinical and Experimental Dentistry, ISSN-e 1989-5488, Vol. 11, Nº. 3 (March ), 2019, págs. 269-274
- Idioma: inglés
- Enlaces
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Resumen
The biological responses involved during retention phase have been studied for many years but little is known about the effect of saliva proteome during retention phase of post-orthodontic treatment. This study aims to identify the protein profiles during retention phase in relation to biological processes involved by Liquid Chromatography Mass Spectrometry (LC-MS) approach.
A total of 5 ml of unstimulated saliva was collected from each subject (10 non-orthodontic patients and 15 post-orthodontic patients with 6-months retention phase). Samples were then subjected to LC-MS analysis. The expressed proteins were identified and compared between groups. Incisor irregularity for both maxilla and mandible were determined with Little’s Irregularity Index at 6-months retention phase.
146 proteins and 135 proteins were expressed in control and 6-months retention phase group respectively. 15 proteins were identified to be co-expressed between groups. Immune system process was only detected in 6-months retention phase group. Detected protein in immune system process was identified as Tyrosine-protein kinase Tec. Statistical significant of incisor irregularity was only found in mandible at 6-months retention phase.
Our study suggests that immune system process protein which is Tyrosine-protein kinase Tec could be used as biomarker for prediction of stability during retention phase of post-orthodontic treatment.
