DNA Barcoding in Forensic Entomology - Establishing a DNA Reference Library of Potentially Forensic Relevant Arthropod Species,

• Chimeno, Caroline ^[1] ; Morinière, Jérôme ^[1] ; Podhorna, Jana ^[2] ; Hardulak, Laura ^[1] ; Hausmann, Axel ^[1] ; Reckel, Frank ^[3] ; Grunwald, Jan E. ^[4] ; Penning, Randolph ^[5] ; Haszprunar, Gerhard ^[1]
  1. [1] Zoologische Staatssammlung München
  2. [2] Mendel University in Brno (MEDELU),
  3. [3] Abteilung II, Bayerisches Landeskriminalamt, Munchem
  4. [4] Abteilung II, Bayerisches Landeskriminalamt, Munchen
  5. [5] Institute of Legal Medicine, Ludwig‐Maximilians Universität, München,

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• Localización: Journal of forensic sciences, ISSN-e 1556-4029, ISSN 0022-1198, Vol. 64, Nº. 2, 2019, págs. 593-601
• Idioma: inglés
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• Resumen

  • Throughout the years, DNA barcoding has gained in importance in forensic entomology as it leads to fast and reliable species determination. High-quality results, however, can only be achieved with a comprehensive DNA barcode reference database at hand. In collaboration with the Bavarian State Criminal Police Office, we have initiated at the Bavarian State Collection of Zoology the establishment of a reference library containing arthropods of potential forensic relevance to be used for DNA barcoding applications. CO1-5P’ DNA barcode sequences of hundreds of arthropods were obtained via DNA extraction, PCR and Sanger Sequencing, leading to the establishment of a database containing 502 high-quality sequences which provide coverage for 88 arthropod species. Furthermore, we demonstrate an application example of this library using it as a backbone to a high throughput sequencing analysis of arthropod bulk samples collected from human corpses, which enabled the identification of 31 different arthropod Barcode Index Numbers.