Resumen de Bioprospecting Reveals Class III ω-Transaminases Converting Bulky Ketones and Environmentally Relevant Polyamines

Cristina Coscolín, Nadine Katzke, Antonio García Moyano, José Navarro-Fernández, David Almendral, Monica Martinez Martinez, Alexander Bollinger, Rafael María Bargiela Bargiela, Christoph Gertler, Tatyana N. Chernikova, David Rojo, Coral Barbas, Hai Tran, Olga V. Golyshina, Rainhard Koch, Michail M. Yakimov, Gro E. K. Bjerga, Peter N. Golyshin, Karl-Erich Jaeger, Manuel Ferrer

• Amine transaminases of the class III ω-TAs are key enzymes for modification of chemical building blocks, but finding those capable of converting bulky ketones and (R) amines is still challenging. Here, by an extensive analysis of the substrate spectra of 10 class III ω-TAs, we identified a number of residues playing a role in determining the access and positioning of bulky ketones, bulky amines, and (R)- and (S) amines, as well as of environmentally relevant polyamines, particularly putrescine. The results presented can significantly expand future opportunities for designing (R)-specific class III ω-TAs to convert valuable bulky ketones and amines, as well as for deepening the knowledge into the polyamine catabolic pathways.