Ayuda
Ir al contenido

Dialnet


Osteoprotegerin mediate RANK/RANKL signaling inhibition eases asthma inflammatory reaction by affecting the survival and function of dendritic cells

  • X. Yang [2] ; X. Wang [2] ; M. Chi [3] ; M. Zhang [2] ; H. Shan [2] ; Q.-H. Zhang [2] ; J. Zhang [2] ; J. Shi [2] ; J.-Z. Zhang [4] ; R.-M. Wu [1] ; Y.-L. Li [2]
    1. [1] Shaanxi Provincial People's Hospital

      Shaanxi Provincial People's Hospital

      China

    2. [2] The Second Affiliated Hospital of Xi’an Jiaotong University, China
    3. [3] BaYi Children's Hospital of the PLA Army General Hospital, China
    4. [4] The First Affiliated Hospital of Xi’an Jiaotong University, China
  • Localización: Allergologia et immunopathologia: International journal for clinical and investigate allergology and clinical immunology, ISSN-e 1578-1267, ISSN 0301-0546, Vol. 47, Nº. 2, 2019, págs. 179-184
  • Idioma: inglés
  • Texto completo no disponible (Saber más ...)
  • Resumen
    • Introduction Asthma is a chronic inflammatory, heterogeneous airway disease affecting millions of people around the world. Dendritic cells (DCs) are considered the most important antigen-presenting cell in asthma airway inflammatory reaction. But whether osteoprotegerin (OPG) mediate RANK/RANKL signaling inhibition influences asthma development by affecting the survival and function of DCs remains unclear. In this study, we assessed the effects of OPG on DCs and asthma.

      Material and methods BALB/c mice immunized with ovalbumin (OVA) were challenged thrice with an aerosol of OVA every second day for eight days. Dexamethasone (1.0 mg/kg) or OPG (50 μg/kg) was administered intraperitoneally to OVA-immunized BALB/c mice on day 24 once a day for nine days. Mice were analyzed for effects of OPG on asthma, inflammatory cell infiltration and cytokine levels in lung tissue. The expression of RANK and β-actin was detected by Western Blot. DCs were isolated from mouse bone morrow. Cell survival was assessed by cell counting. The content of IL-12 was detected by ELISA.

      Results Results showed that OVA increased the number of inflammatory factors in BALF, elevated lung inflammation scores in mice. OPG reversed the alterations induced by OVA in the asthmatic mice. OPG inhibited the survival and function of DC via inhibition of RANK/RANKL signaling.

      Conclusions This research proved inhibition of RANK/RANKL signaling by OPG could ease the inflammatory reaction in asthma, providing new evidence for the application of OPG on asthma.


Fundación Dialnet

Dialnet Plus

  • Más información sobre Dialnet Plus

Opciones de compartir

Opciones de entorno