Resumen de Presencia del sugarcane yellow leaf virus (SCYLV) en caña de azúcar (Saccharumspp.) aislado de Colima

María Inés Barbosa Villa, José Luis Cruz Jaramillo, Hilda Victoria Silva Rojas, Karina de la Paz García Mariscal, José Concepción García Preciado, Manuel de Jesús Bermúdez Guzmán

• Multiple

  Lacaña de azúcar es uno de los cultivos industriales importantes a nivel mundial y es afectado por diversas enfermedades virales, incluidoelsugarcane yellow leafvirus(SCYLV). En México este virus se ha detectado basado en sintomatologíay °Brix;sin embargo, el diagnóstico basado en estos parámetros no es concluyente. Elobjetivo del estudiofue detectar la presencia y distribucióndel SCYLVen zonas cañerasdel Occidentede México(Colima, Jalisco y Nayarit)y determinar elorigenfilogenéticode un aisladode Colima.El trabajo se desarrolló en el Campo Experimental Tecomán durante 2013-2014.Se aisló elRNA totala partir de hojascolectadas y se realizaron las RT-PCR con oligonucleótidos específicos para SCYLV.Se analizaron 233muestras y la incidencia de plantas portadoras del SCYLV fue 14.6%,afectando a los híbridosCP 72-2086, Mex 69-290 y Atemex 96-40. Elanálisis BLAST mostróque la secuenciaparcial CPdeColmex-317 (512 pb) es homóloga con secuencias de Brasil, China, India, Kenia y USA,además comparte porcentajes de identidad superiora99% con otras secuencias provenientes de varios países. El análisis filogenético deColmex-317con secuencias parciales y genomas completos del SCYLV aislados de diversas partes del mundo reveló que el aislado mexicano pertenece al genotipo de Brasil (BRA)y se agrupó con secuencias deBrasil, China, Kenia y Sudáfrica. Sin embargo, es necesario evaluar un mayor número de aislados ysecuencias de mayor longitudpara determinar si el genotipo BRA es el único presente en México.

• English

  Sugarcane isone of the important industrial crops worldwide and is affected by various viral diseases, including the Sugarcane yellow leaf virus (SCYLV). In Mexico, this virus has been detected based on symptomatology and °Brix; however, the diagnosis based on these parameters is not conclusive. The objective of the study was to detect the presence and distribution of SCYLV in sugarcane areas of western Mexico (Colima, Jalisco and Nayarit) and to determine the phylogenetic origin of a Colima isolate. The work was developed in the Tecoman Experimental Field during 2013-2014. Total RNA was isolated from collected leaves and RT-PCR was performed with oligonucleotides specific for SCYLV. A total of 233 samples were analyzed and the incidence of SCYLV carrier plants was 14.6%, affecting the hybrids CP 72-2086, Mex 69-290 and Atemex 96-40. The BLAST analysis showed that the partial CP sequence of Colmex-317 (512 bp) is homologous with sequences from Brazil, China, India, Kenya and USA and also shares identity percentages higher than 99% with other sequences from several countries. The phylogenetic analysis of Colmex-317 with partial sequences and complete genomes of SCYLV isolated from different parts of the world revealed that the Mexican isolate belongs to the Brazilian genotype (BRA) and was grouped with sequences from Brazil, China, Kenya and South Africa. However, it is necessary to evaluate a larger number of isolates and longer sequences to determine if the BRA genotype is the only one present in Mexico.