Versatile Fungal Polyphenol Oxidase with Chlorophenol Bioremediation Potential: Characterization and Protein Engineering

• Efstratios Nikolaivits ^[1] ; Maria Dimarogona ^[2] ; Ioanna Karagiannaki ^[1] ; Angelina Chalima ^[1] ; Ayelet Fishman ^[3] ; Evangelos Topakas ^[1]
  1. [1] National Technical University of Athens

     National Technical University of Athens

     Dimos Athens, Grecia

     
  2. [2] University of Patras

     University of Patras

     Dimos Patras, Grecia

     
  3. [3] Technion – Israel Institute of Technology

     Technion – Israel Institute of Technology

     Israel

     

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• Localización: Applied and Environmental Microbiology, ISSN 0099-2240, Vol. 84, Nº 23, 2018
• Idioma: inglés
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• Resumen

  • A novel fungal PPO was heterologously expressed and biochemically characterized. Construction of single and double mutants led to the generation of variants with altered specificity against CPs. Through this work, knowledge is gained regarding the effect of mutations on the substrate specificity of PPOs. This work also demonstrates that more potent biocatalysts for the bioremediation of harmful CPs can be developed by applying site-directed mutagenesis.