Genomic Characterization of Cyanophage vB_AphaS-CL131 Infecting Filamentous Diazotrophic Cyanobacterium Aphanizomenon flos-aquae Reveals Novel Insights into Virus-Bacterium Interactions

• Sigitas Šulčius ^[4] ; Eugenijus Šimoliūnas ^[5] ; Gediminas Alzbutas ^[6] ; Giedrius Gasiūnas ^[7] ; Vykintas Jauniškis ^[8] ; Jolita Kuznecova ^[1] ; Sini Miettinen ^[2] ; Emelie Nilsson ^[3] ; Rolandas Meškys ^[5] ; Elina Roine ^[2] ; Ričardas Paškauskas ^[1] ; Karin Holmfeldt ^[3]
  1. [1] Nature Research Centre

     Nature Research Centre

     Lituania

     
  2. [2] University of Helsinki

     University of Helsinki

     Helsinki, Finlandia

     
  3. [3] Linnaeus University

     Linnaeus University

     Suecia

     
  4. [4] a Laboratory of Algology and Microbial Ecology, Nature Research Centre, Vilnius, Lithuania; b Center for Ecology and Evolution in Microbial Model Systems, Department of Biology and Environmental Science, Linnaeus University, Kalmar, Sweden
  5. [5] c Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania
  6. [6] a Laboratory of Algology and Microbial Ecology, Nature Research Centre, Vilnius, Lithuania; d Thermo Fisher Scientific Baltics, Vilnius, Lithuania; e Faculty of Mathematics and Informatics, Vilnius University, Vilnius, Lithuania
  7. [7] f Department of Protein-DNA Interactions, Institute of Biotechnology, Vilnius University, Vilnius, Lithuania; j CasZyme, Vilnius, Lithuania
  8. [8] f Department of Protein-DNA Interactions, Institute of Biotechnology, Vilnius University, Vilnius, Lithuania; g Protein Structure and Function Programme, Structural Molecular Biology Group, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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• Localización: Applied and Environmental Microbiology, ISSN 0099-2240, Vol. 85, Nº 1, 2019
• Idioma: inglés
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  • The genomic characterization of novel cyanophage vB_AphaS-CL131 and the analysis of its genomic features in the context of other viruses, metagenomic data, and host CRISPR-Cas systems contribute toward a better understanding of aquatic viral diversity and distribution in general and of brackish-water cyanophages infecting filamentous diazotrophic cyanobacteria in the Baltic Sea in particular. The results of this study revealed previously undescribed features of cyanophage genomes (e.g., self-excising intein-containing putative dCTP deaminase and putative cyanophage-encoded CRISPR-Cas and toxin-antitoxin systems) and can therefore be used to predict potential interactions between bloom-forming cyanobacteria and their cyanophages.