Genotype and Phenotype of Caffeine Metabolism: A Biochemistry Laboratory Experiment

• Autores: Julie T. Millard, Tenzin Passang, Jiayu Ye, Gabriel M. Kline, Tina M. Beachy, Victoria L. Hepburn, Edmund J. Klinkerch
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 95, Nº 10, 2018, págs. 1856-1860
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • An experiment for the upper-division undergraduate biochemistry laboratory is described in which students investigate the influence of genetic variations of cytochrome P450 1A2 on drug metabolism, using caffeine as a model compound. Saliva samples from human subjects are characterized for a single-nucleotide polymorphism in the CYP1A2 gene (genotyping) and for the rate of caffeine clearance (phenotyping). This experiment has clinical significance in the area of personalized medicine.