Two experiments were implemented using three cannulated crossbreed rams (Corriedale × Suffolk) fed rice straw-based diet. In vivo evaluation of garlic powder (GP) supplementation to rice straw-based diet on CH4 and CO2 emissions and blood profiles of the rams (body weight = 52 ± 3 kg) was performed. The design was a triplicate 3 × 3 Latin square. Groups were the control (no GP) and two GP groups, which were given supplementation for 0.1 (GP0.1) and 0.5% (GP0.5) of concentrate. The differences between two experiments were data missing of GP0.1 and uncontrolled environment in Experiment 1. Blood profiles were added in Experiment 2.; Results: In Experiment 1, supplementation of GP to the rams reduced CH4 production significantly (P < 0.05) by 38%. Dry matter intake was not different among the groups in both experiments. In Experiment 2, CH4 was lower in the GP0.5 group than the control group by 7%, whereas CO2 showed no difference among the groups. Serum glutamic oxaloacetic transaminase was significantly (P > 0.05) lower in the GP0.5 group than the control group, whereas glutamic pyruvic transaminase, total protein, urea N, total cholesterol, low-density lipoprotein, calcium, phosphorus, magnesium and non-esterified fatty acids were not significantly (P > 0.05) different.; Conclusions: GP supplementation, particularly GP0.5, decreases CH4 emissions in rams fed rice straw-based diets. © 2018 Society of Chemical Industry.; © 2018 Society of Chemical Industry.
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