Effect of harvest time on the levels of phytochemicals, free radical-scavenging activity, α-amylase inhibition and bile acid-binding capacity of spinach (Spinacia oleracea).
- Autores: Noorani Barkat, Jashbir Singh, Guddadarangavvanahally K Jayaprakasha, Bhimanagouda S. Patil
- Localización: Journal of the science of food and agriculture, ISSN 0022-5142, Vol. 98, Nº 9, 2018, págs. 3468-3477
- Idioma: inglés
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Resumen
Spinach is a green leafy vegetable that is rich in health-promoting compounds. The present study analyzed the levels of phytochemicals and health-promoting properties of spinach harvested at 20, 30, 40, 50 and 60 days after planting.; Results: The time of harvest had a significant effect on nitrate levels, which increased from 1909 ± 70.6 µg g-1 (20 days) to 3668 ± 101.3 µg g-1 (40 days) and then decreased to 974 ± 164 µg g-1 (60 days). Lutein and chlorophylls a and b were found to be maximum at 60 days, whereas β-carotene was higher at 50 days. Liquid chromatography/high-resolution quadrupole time-of-flight tandem mass spectrometry (LC/HR-QTOF-MS) was used to identify 12 flavonoids, and their tentative fragmentation pathways have been proposed. Spinach harvested at 30 and 60 days exhibited significantly higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical-scavenging activities and inhibition of amylase. The levels of total phenolics ranged from 885 ± 35.1 to 1162 ± 112.4 µg g-1 in the samples. In vitro bile acid-binding capacity showed that glycochenodeoxycholate and glycodeoxycholate were bound to maximum levels in all spinach samples.; Conclusion: The harvest time has a major effect on the levels of phytochemicals and health-beneficial properties, which indicates that consumption of both baby and mature spinach will provide maximum health benefits. © 2017 Society of Chemical Industry.; © 2017 Society of Chemical Industry.
