Effect of high chronic intake of sucrose on liver metabolism in aging rats. Modulation by rutin and micronutrients

• Eva Gatineau ^[1] ; Frédéric Capel ^[1] ; Dominique Dardevet ^[1] ; Jérémie David ^[1] ; Corinne Pouyet ^[1] ; Sergio Polakof ^[1] ; Laurent Mosoni ^[1]
  1. [1] University of Clermont Auvergne

     University of Clermont Auvergne

     Arrondissement de Clermont-Ferrand, Francia

     
• Localización: Journal of physiology and biochemistry, ISSN-e 1877-8755, ISSN 1138-7548, Vol. 74, Nº. 4 (November 2018), 2018 (Ejemplar dedicado a: CTPIOD meeting (Contribution To Progress in Obesity and Diabetes Research) 2017 / Fermín Ignacio Milagro Yoldi (ed. lit.), Christian Carpéné (ed. lit.)), págs. 569-577
• Idioma: inglés
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• Resumen

  • High-sugar intake and senescence share common deleterious effects, in particular in liver, but combination of these two factors was little studied. Our aims were to examine the effect of a high-sucrose diet in liver of old rats and also the potential benefices of a polyphenol/micronutrient supplementation. Four groups of 22-month-old male rats fed during 5 months with a diet containing either 13 or 62% sucrose, supplemented or not with rutin, vitamin E, A, D, selenium, and zinc were compared. We measured liver macronutrient composition, glycation/oxidative stress, enzyme activities (lipogenesis, β-oxidation, fructokinase), gene expression (enzymes and transcription factors), in vivo protein synthesis rates and plasma parameters. Sucrose induced an increase in plasma and liver lipid content, and a stimulation of liver protein synthesis rates. Gene expression was little changed by sucrose, with lower levels for LXR-α and LXR-β. Polyphenol/micronutrient supplementation tended to limit liver triglyceride infiltration through variations in fatty acid synthase, acyl coA oxidase, and possibly ATP-citrate lyase activities. In conclusion, despite differences in enzymatic regulations, and blunted responses of gene expression, high-sucrose diet was still able to induce a marked increase in liver lipid content in old animals. However, it probably attenuated the positive impact of polyphenol/micronutrients.