Determination of S‐methyl‐L‐methionine (SMM) from BrassicaceaeFamily Vegetables and Characterization of the Intestinal Transport of SMM by Caco‐2 Cells

• Autores: Ji‐Hoon Song, Hae‐Rim Lee, Soon‐Mi Shim
• Localización: Journal of food science, ISSN 0022-1147, Vol. 82, Nº 1, 2017, págs. 36-43
• Idioma: inglés
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• Resumen

  • The objectives of the current study were to determine S‐methyl‐L‐methionine(SMM) from various Brassicaceaefamily vegetables by using validated analytical method and to characterize the intestinal transport mechanism of SMM by the Caco‐2 cells. The SMM is well known to provide therapeutic activity in peptic ulcers. The amount of SMM from various Brassicaceaefamily vegetables ranged from 89.08 ± 1.68 μg/g to 535.98 ± 4.85 μg/g of dry weight by using validated ultra‐performance liquid chromatography‐electrospray ionization‐mass spectrometry method. For elucidating intestinal transport mechanism, the cells were incubated with or without transport inhibitors, energy source, or a metabolic inhibitor. Phloridzin and verapamil as inhibitors of sodium glucose transport protein (SGLT1) and P‐glycoprotein, respectively, were not responsible for cellular uptake of SMM. Glucose and sodium azide were not affected by the cellular accumulation of SMM. The efflux ratio of SMM was 0.26, implying that it is not effluxed through Caco‐2 cells. The apparent coefficient permeability (Papp) of SMM was 4.69 × 10−5cm/s, indicating that it will show good oral absorption in in vivo. The data from this study reveal a practical application worthy of future study. It would be valuable to develop value added functional ingredient for supporting health promotion by using vegetables containing SMM or SMM concentrates.