Resumen de Antimicrobial Effects of Silver Nanoparticles Synthesized by Fatsia japonicaLeaf Extracts for Preservation of CitrusFruits
Jing Zhang, Guoguo Si, Jun Zou, Ruiliang Fan, Ailing Guo, Xuetuan Wei
Due to their potent antimicrobial activity, silver nanoparticles (AgNPs) synthesized by biomass might be promising in food preservation, whereas effects of AgNPs on Penicillium italicum‐induced rot of Citrusfruits have not been investigated. In this study, a novel AgNPs biosynthesis method was developed based on Fatsia japonicaleaf extracts. It was revealed that concentrations of leaf extracts, AgNO3and NaCl affected AgNPs yields and particle sizes obviously. Under the optimized conditions (8 mg/mL extracts, 2 mM AgNO3and 1 mM NaCl), AgNPs, synthesized within 80 min, showed potent preservative effect against P. italicum‐induced rot of Citrusfruits. Furthermore, inhibition test and TEM analysis indicated that as‐synthesized AgNPs caused cell deformation, cytoplasmic leakage, and thereupon cell death of P. italicum. Moreover, AgNPs had significant antibacterial activities against Escherichia coliand Staphylococcus aureus, which might be beneficial for Citrusfruits preservation. Altogether this study develops an efficient AgNPs synthesis method and a novel preservation method for Citrusfruits. The AgNPs biosynthesis method mediated by Fatsia japonicaleaf extracts has the potential for large scale production of AgNPs. Moreover, as‐synthesized AgNPs can be further used in preservation of Citrusfruits.
