Physiological Changes of Surface Membrane in Lactobacilluswith Prebiotics

• Autores: Mingfang Pan, Kishore K. Kumaree, So‐Hyun Park
• Localización: Journal of food science, ISSN 0022-1147, Vol. 82, Nº 3, 2017, págs. 744-750
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Synbiotics are always considered to be beneficial in healthy manipulation of gut environment; however, the purpose of this research was to investigate the dominance of synbiotic over the individual potential of probiotics and prebiotics. Four different types of prebiotics, fructo‐oligosaccharides, raffinose, inulin, and cellobiose, were evaluated based on their varying degree of polymerization, combined each with 2 different Lactobacillistrains, including Lactobacillus paracasei276 and Lactobacillus plantarumWCFS1. The effects of synbiotics combination on the surface structure were evaluated by analyzing auto‐aggregation, membrane hydrophobicity, and adhesion to Caco‐2 cells. Our results showed that both Lactobacilliexhibited significantly greater degree of attachment to Caco‐2 cells (23.31% and 16.85%, respectively) when using cellobiose as a substrate than with other prebiotics (P< 0.05). Intestinal adhesion ability was in correlation with the percent of auto‐aggregation, both Lactobacillusexhibited higher percent of auto‐aggregation in cellobiose compared to other prebiotics. These behavioral changes in terms of attachment and auto‐aggregation were further supported with the changes noticed from infrared spectra (FT‐IR).