Resumen de Effect of In VitroDigestion on the Total Antioxidant Capacity and Phenolic Content of 3 Species of Oregano (Hedeoma patens, Lippia graveolens, Lippia palmeri)

Erick Paul Gutiérrez‐Grijalva, Miguel Angel Angulo‐Escalante, Josefina León-Félix, J.B. Heredia

• Oregano phenolic compounds have been studied for their anti‐inflammatory properties. Nonetheless, after ingestion, the gastrointestinal environment can affect their antioxidant stability and thus their bioactive properties. To evaluate the effect of in vitrogastrointestinal (GI) digestion on the phenolic compounds of 3 species of oregano (Hedeoma patens, Lippia graveolens, and Lippia palmeri), the total reducing capacity, total flavonoid content, and antioxidant capacity were evaluated before and after in vitroGI digestion. In addition, the phenolic compounds of the 3 oregano species were identified and quantified by UPLC‐PDA before and after in vitroGI digestion. It was shown that the reducing capacity, flavonoid content and antioxidant capacity were affected by the GI digestion process. Moreover, the phenolic compounds identified were apigenin‐7‐glucoside, scutellarein, luteolin, luteolin‐7‐glucoside, phloridzin and chlorogenic acid, and their levels were affected by the in vitroGI process. Our results showed that the phenolic compounds from these 3 species of oregano are affected by the in vitrodigestion process, and this effect is largely attributable to pH changes. These changes can modify the bioavailability and further anti‐inflammatory activity of oregano phenolics, and thus, further research is needed. Oregano is a rich source of polyphenols that have shown bioactive properties like anti‐inflammatory potential. However, little is known of the gastrointestinal fate of oregano polyphenols which is imperative to fully understand its bioaccessibility. Our results are important to develop new administration strategies which could help protect the antioxidant and anti‐inflammatory potential and bioaccessibility of such compounds.