Detection of Enterotoxin Genes and Methicillin‐Resistance in Staphylococcus aureusIsolated from Water Buffalo Milk and Dairy Products

• Autores: Erdem Saka, Goknur Terzi Gulel
• Localización: Journal of food science, ISSN 0022-1147, Vol. 83, Nº 6, 2018, págs. 1716-1722
• Idioma: inglés
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• Resumen

  • The aim of this study was to analyze the presence of genes encoding staphylococcal enterotoxins and methicillin resistance in Staphylococcus aureusisolates obtained from water buffalo milk and dairy products. A total of 200 samples (100 raw milk, 50 clotted cream, and 50 cheese samples) was collected from different dairy farms and smallholders in Samsun, Turkey. All samples were analyzed using the standard procedure EN ISO 6888‐1 and isolates were confirmed for the presence of the target 16S rRNA specific for Staphylococcusgenus specific and nucgene specific for S. aureusspecies by PCR. S. aureuswas identified in 30 of 100 milk (30%), 9 of 50 clotted cream (18%), and 17 of 50 cheese (34%) samples. A total of 99 isolates was confirmed as S. aureus. Genotypic methicillin resistance was evaluated using PCR for the mecAgene. Out of 99 isolates, nine (9%) were found to be methicillin resistant (mecAgene positive). Twelve out of 99 (12%) S. aureusisolates were found positive for one or more genes encoding the enterotoxins. The gene coding for enterotoxin, sea, was the most frequent (five isolates, 41.6%), followed by sec(two isolates, 16.6%), sed(1 isolates, 8.3%) and see(1 isolate, 8.3%). While three isolates (25%) contained both secand sed, none of the samples was positive for seb. In conclusion, the presence of segene‐positive and methicillin‐resistant S. aureusin buffalo milk and products revealed that consumption of these products is a potential risk of foodborne infection in this region. Enterotoxigenic and methicillin‐resistant S. aureus(MRSA) in milk and dairy products is an important public health problem. Especially in traditional dairy products, Staphylococcal enterotoxins may cause food poisoning due to consumption of raw or unpasteurized milk products.