Identification of Free Radical Scavengers from Brazilian Green Propolis Using Off‐Line HPLC‐DPPH Assay and LC‐MS
- Autores: Cuiping Zhang, Xiaoge Shen, Jiawei Chen, Xiasen Jiang, FuLiang Hu
- Localización: Journal of food science, ISSN 0022-1147, Vol. 82, Nº 7, 2017, págs. 1602-1607
- Idioma: inglés
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Resumen
Brazilian green propolis is known as an appreciable natural antioxidant with abundant polyphenolic compounds. For quality control, a fingerprint‐efficacy study of Brazilian green propolis was carried out in this work. Chemical fingerprints of Brazilian green propolis from 22 different sources were determined by HPLC and investigated by similarity analysis. The fingerprint‐efficacy relationships between chemical fingerprint and DPPH radical‐scavenging activity were established. The results showed that 14 characteristic common peaks were identified, and 9 compounds were discovered with free radical‐scavenging activities. Caffeoylquinic acids and artepillin C might be the major effective components for quality control of Brazilian green propolis due to their specificity and strong antioxidant activity. This study provides new markers for the quality assessment of Brazilian green propolis and its derived products.
