Stereological and ultrastructural quantification of the afferent synaptome of individual neurons.

• Autores: Pablo Henny, Matthew T C Brown, Benjamin R Micklem, Peter J Magill, J Paul Bolam
• Localización: Brain Structure and Function, ISSN 1863-2653, ISSN-e 1863-2661, Vol. 219, Nº. 2, 2014, págs. 631-640
• Idioma: inglés
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• Resumen

  • Determining the number and placement of synaptic inputs along the distinct plasma membrane domains of neurons is essential for explaining the basis of neuronal activity and function. We detail a strategy that combines juxtacellular labeling, neuronal reconstructions and stereological sampling of inputs at the ultrastructural level to define key elements of the afferent 'synaptome' of a given neuron. This approach provides unbiased estimates of the total number and somato-dendritic distribution of synapses made with individual neurons. These organizational properties can be related to the activity of the same neurons previously recorded in vivo, for direct structure-function correlations at the single-cell level. The approach also provides the quantitative data required to develop biologically realistic models that simulate and predict neuronal activity and function.;