Multilocus Variable-Number Tandem-Repeat Analysis of Yersinia ruckeri Confirms the Existence of Host Specificity, Geographic Endemism, and Anthropogenic Dissemination of Virulent Clones

• Snorre Gulla ^[2] ; Andrew C. Barnes ^[3] ; Timothy J. Welch ^[4] ; Jesús L. Romalde ^[1] ; David Ryder ^[5] ; Michael J. Ormsby ^[6] ; Jeremy Carson ^[7] ; Karin Lagesen ^[2] ; David W. Verner-Jeffreys ^[5] ; Robert L. Davies ^[6] ; Duncan J. Colquhoun ^[8]
  1. [1] Universidade de Santiago de Compostela

     Universidade de Santiago de Compostela

     Santiago de Compostela, España

     
  2. [2] aNorwegian Veterinary Institute, Oslo, Norway
  3. [3] bUniversity of Queensland, Brisbane, Australia
  4. [4] cUSDA-ARS-NCCCWA, Leetown, West Virginia, USA
  5. [5] eCefas, Weymouth Laboratory, Weymouth, England
  6. [6] fUniversity of Glasgow, Glasgow, Scotland
  7. [7] gDepartment of Primary Industries, Parks, Water and Environment, Hobart, Tasmania, Australia
  8. [8] aNorwegian Veterinary Institute, Oslo, Norway; hUniversity of Bergen, Bergen, Norway

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• Localización: Applied and Environmental Microbiology, ISSN 0099-2240, Vol. 84, Nº 16, 2018
• Idioma: inglés
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  • This comprehensive population study substantially improves our understanding of the epizootiological history and nature of an internationally important fish-pathogenic bacterium. The MLVA assay developed and presented represents a high-resolution typing tool particularly well suited for Yersinia ruckeri infection tracing, selection of strains for vaccine inclusion, and risk assessment. The ability of the assay to separate isolates into geographically linked and/or possibly host-specific clusters reflects its potential utility for maintenance of national biosecurity. The MLVA is internationally applicable and robust, and it provides clear, unambiguous, and easily interpreted results. Typing is reasonably inexpensive, with a moderate technological requirement, and may be completed from a harvested colony within a single working day. As the resulting MLVA profiles are readily portable, any Y. ruckeri strain may rapidly be placed in a global epizootiological context.