Teaching Undergraduates LC–MS/MS Theory and Operation via Multiple Reaction Monitoring (MRM) Method Development
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Thomas A. Betts [1] ; Julie A. Palkendo [1]
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[1] Kutztown University
Kutztown University
Borough of Kutztown, Estados Unidos
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- Localización: Journal of chemical education, ISSN 0021-9584, Vol. 95, Nº 6, 2018, págs. 1035-1039
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
This laboratory experiment employs an innovative approach to introduce undergraduates to liquid chromatography–tandem mass spectrometry (LC–MS/MS) by developing a method based on multiple reaction monitoring (MRM). During this qualitative investigation, students operated a triple quadrupole mass spectrometer in the MS scan and product ion scan modes to determine precursor and product ions, respectively, for a vanillin, ethyl vanillin, and coumarin standard mixture. After identifying precursor → product ion transitions, students conducted a 2 min liquid chromatography run in MRM mode. Students were astonished by the speed, selectivity, and sensitivity of the LC–MS/MS method compared to that of a liquid chromatograph with ultraviolet detection.
