Topically applied azaphenothiazines inhibit contact sensitivity to oxazolone in mice

• Jolanta Artym ^[1] ; Maja Kocięba ^[1] ; Ewa Zaczyńska ^[1] ; Michał Zimecki ^[1] ; Małgorzata Jeleń ^[2] ; Beata Morak-Młodawska ^[2] ; Krystian Pluta ^[2] ; Katarzyna Kaleta-Kuratewicz ^[3] ; Jan P. Madej ^[3] ; Piotr Kuropka ^[3] ; Jan Kuryszko ^[3]
  1. [1] Polish Academy of Sciences

     Polish Academy of Sciences

     Warszawa, Polonia

     
  2. [2] Medical University of Silesia

     Medical University of Silesia

     Katowice, Polonia

     
  3. [3] Wrocław University, Poland

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• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 33, Nº. 2, 2018, págs. 223-236
• Idioma: inglés
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  • In this work we investigated the efficacy of two topically applied azaphenothiazine derivatives, 9- c h l o r o - 6 - a c e t y l ami n o b u t y l q u i n o b e n z o [ 3 , 2 - b][1,4]thiazine (compound 4) and 6-chloroethylureidoethyldiquino[3,2-b;2’;3’-e][1,4]thiazine (compound 5), in the amelioration of inflammatory symptoms of contact sensitivity (CS) to oxazolone in mice, in relation to the commercial ointment Protopic® (tacrolimus), the reference drug. The compounds were administered 24 h following elicitation of CS and, 24 h later, the parameters of inflammation, such as ear edema, blood composition, leukocyte level, numbers of cells in the draining lymph nodes, histological picture of the inflamed tissue, and the morphometric analysis, were analyzed. The study showed that the effectiveness of the studied azaphenothiazines applied as a 0.1% ointment was comparable to the reference drug regarding suppression of the inflammatory process, when all the investigated histological parameters are taken into account.