Identification of 100 KDa protein in sera of micetreated with Cu(II) complex with superoxide dismutase-mimetic activity

• Autores: M. M. El-Naggar, OmAli Y. El-Khawaga
• Localización: Journal of physiology and biochemistry, ISSN-e 1877-8755, ISSN 1138-7548, Vol. 59, Nº. 1 (march), 2003, págs. 35-42
• Idioma: inglés
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• Resumen
  • español

    Se analiza mediante SDS-PAGE el perfil protéico de suero sanguíneo de ratón tras el tratamiento con el complejo de Cu(II) de Girard T, con actividad superóxido dismutasa (SOD). El complejo se administra (ip, 100 mg/Kg peso corporal) a ratones swiss albinos. Tras electrofóresis, se obtiene una nueva banda polipeptídica de 100 Ka que se utiliza para inmunizar conejos y obtener el correspondiente anticuerpo policlonal. El suero de conejo antiproteíco de 100 KDa permite detectar con precisión la presencia de esa proteína en suero de ratón tratado, lo que no ocurre con el no tratado. Mediante diferentes métodos, se confirma la indentificación y se caracteriza el polipéptido purificado

  • English

    The protein profile of sera isolated from mice pre-treated with Cu(II) complex of Girard T with superoxide dismutase (SOD)-mimetic activity was analyzed using SDS-PAGE. This complex was intraperitoneally administered (10 mg/Kg body weight) to Swiss albino mice. The resolved polypeptides showed a new sharp band at 100 Kda against which a polyclonal antibody was raised in rabbit. Sera of rabbit anti-100 KDa protein was used as a powerful probe for the detection of 100 KDa protein isolated from sera of treated mice. Western blot assays revealed a strong reactive polypeptide band at 100 KDa in sera of the mice, but no cross reaction was observed with sera of normal mice. The identification of purified polypeptide was confirmed by different characterization experiments