Making Enzyme Kinetics Dynamic via Simulation Software
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[1] Concordia University
Concordia University
Canadá
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- Localización: Journal of chemical education, ISSN 0021-9584, Vol. 95, Nº 3, 2018, págs. 482-486
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
An interactive classroom demonstration that enhances students’ knowledge of steady-state and Michaelis–Menten enzyme kinetics is described. The instructor uses a free version of professional-quality KinTek Explorer simulation software and student input to construct dynamic versions of three static hallmark images commonly used to introduce enzyme kinetic concepts. The software, with its ability to change experimental conditions and immediately observe the effects on the kinetics of a system, allows students to be more aware of the experimental conditions that must exist for the assumptions of steady-state and Michaelis–Menten kinetics to be valid. Students report an increased understanding of the interplay between hallmark concentration versus time and rate versus concentration images. After the demonstration, students are prepared for data generation and analysis in a lab experiment focused on the steady-state. Additionally, they can begin independent simulation exercises using the software and are ready for discussions on pre-steady-state kinetics.
