Morphological description and molecular detection of Pestalotiopsis sp. on hazelnut in Serbia

• Tanja Vasić ^[2] ; Darko Jevremović ^[3] ; Vesna Krnjaja ^[4] ; Aleksandar Leposavić ^[3] ; Snežana Anđelković ^[2] ; Sanja Živković ^[1] ; Svetlana Paunović ^[3]
  1. [1] University of Belgrade

     University of Belgrade

     Serbia

     
  2. [2] Institute for Forage Crops, Serbia
  3. [3] Fruit Research Institute, Serbia
  4. [4] Institute for Animal Husbandry, Serbia

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• Localización: Spanish journal of agricultural research, ISSN-e 2171-9292, ISSN 1695-971X, Vol. 15, Nº. 3, 2017
• Idioma: inglés
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• Resumen

  • In autumn 2015, hazelnut plants with leaf blight symptoms were noticed in a commercial plantation in the Province of Vojvodina, Serbia.

    Symptomatic samples were collected and submitted to laboratory analysis. Based on morphological characterization, the fungus isolated from the material was initially identified as Pestalotiopsis sp. Pathogenicity tests showed that two selected isolates infected hazelnut leaves and fruits that developed symptoms after artificial inoculation. The pathogen was re-isolated from diseased leaves and fruits, confirming Koch’s postulates. Molecular identification was performed with sequence and phylogenetic analysis of ITS, EF1-α, and TUB genomic regions.

    Phylogenetic analysis confirmed the results of the morphological identification. The detection of Pestalotiopsis sp., a causal agent of leaf blight on hazelnut in Serbia, is one of a few reports of these pathogenic fungi on hazelnut.