The antimicrobial activities of anethole, anisic acid, and eugenol characterized from aniseed were tested against 18 organisms including both bacteria and yeasts. As far as their minimum inhibitory concentration (MIC) values were compared, they are nearly comparable but act in different ways. For example, anethole was noted to be effective against Saccharomyces cerevisiae with a minimum fungicidal concentration (MFC) of 200 µg mL−1 but the activity was observed only when this yeast was growing on fermentable carbon sources in a hypoxic condition. On the other hand, eugenol was effective against S. cerevisiae with an MFC of 800 µg mL−1 in any growing conditions. Anisic acid showed fungistatic activity against this yeast with an MIC of 400 µg mL−1, but not fungicidal up to 1600 µg mL−1. Copyright © 2007 Society of Chemical Industry
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