Development of high-performance liquid chromatography and non-aqueous capillary electrophoresis methods for the determination of fenoxycarb residues in wheat samples

• Autores: Mian Liu, Hui Yang, Hongxia Liu, Po Han, Xie Wang, Shusheng Zhang, Yangjie Wu
• Localización: Journal of the science of food and agriculture, ISSN 0022-5142, Vol. 88, Nº 1, 2008, págs. 62-67
• Idioma: inglés
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• Resumen

  • BACKGROUND: Practical methods for the analysis of fenoxycarb residues in wheat samples were developed using high-performance liquid chromatography (HPLC) and non-aqueous capillary electrophoresis (NACE). RESULTS: Fenoxycarb residues in wheat were extracted with acetone by ultrasonication, followed by a clean-up procedure with liquid–liquid extraction with 5% NaCl/dichloromethane. The HPLC was developed using C18 as column, MeOH/water (6:4, v/v) as the mobile phase and 199 nm as the detection wavelength. The optimal NACE condition was established with the running buffer of 20.0 mmol L−1 NH4Ac in 95% MeOH (pH* 9.0), and the applied voltage of 30 kV over a capillary of 50 µm i.d. × 48.5 cm × 40 cm effective length. Both methods gave the relatively lower limits of detection (0.008 mg kg−1 for HPLC and 0.024 mg kg−1 for NACE) and the higher recoveries (>85.0%). They were successfully applied to the determination of fenoxycarb in wheat samples. CONCLUSION: The results showed that the fenoxycarb residue gradually reduced to trace amounts after about 3 years, which implied that the pharmacological actions of fenoxycarb could last for about 3 years. Meanwhile, more effort should be made to control and reduce fenoxycarb residues because of its potential health risks to consumers. Copyright © 2007 Society of Chemical Industry